Tris-Saline Buffer Concentrate
REF
VBC-20
Diluted buffer fulfills the USP and Ph. Eur. requirements for Anti-factor Xa and Anti-
factor IIa heparin sodium assay buffer. The buffer is intended for the reconstitution of freeze-dried (lyophilized) enzyme factors (Xa and IIA) and Antithrombin, and can also be used as a diluent in the final assay solution.
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Each vial of VBC-20 contains 20 ml of aqueous 5X Buffer Concentrate with the following composition:
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Tris-base
-
Sodium chloride
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Disodium EDTA
-
PEG-6000 (macrogol)
-
pH 8.4 (working solution, at 20°C)
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Product should be stored refrigerated (2-8°C) and is stable until expiry in the unopened state. Stable 6 months at 2-8°C once diluted. Shipment of unopened product does not require refrigeration.
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Prior to use, the entire contents (20 ml) of VBC-20 should be diluted to a 1X working solution by addition of 4 parts (80 ml) of purified water.
0.250 M
0.875 M
0.0375 M
0.5% (w/v)
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